Phenotypic and Genotypic Characterization of Salmonella Spp.isolated From broiler chickens and Human

Abstract

The aim of this present study to survey the antimicrobial resistance, ESβL and virulence genes among Salmonella serovars isolated from broiler chickens and Human .300 broiler chickens and 60 stool human samples were investigated for Salmonella by cultural, biochemical and serotyping, 44 isolates were positive for Salmonella with the most predominant serotypes are S. Enteritidis and S. Typhimurium in all isolates from broilers chickens and Human. Broilers Salmonella isolates showed high resistance to Imipenem (83.3%) followed by Ceftriaxone(73.3%),On contrast showed high sensitive to Cephalexin (73.3%).But in human isolates showed high resistance to Ampicillin-Sulbactam (21.4%) ,On contrast showed high sensitive to Ceftazidime, Amikacin and Trimethoprim-Sulfamethoxazole (100 %) for each . By using PCR test for detection of four virulence genes ( invA , adrA , ompA and csgD) and nine resistance genes ( int1, int2, int3, BlaTEM, BlaCTX, BlaOXA, Mox,gyrA and gyrS), detected the presence of invA virulence and BlaTEM resistance gene in all serovars isolated from broiler chickens and human. Finally, in our study the results of genotypic and phenotypic analysis, found close relation between human and broiler chickens Salmonella strains.