Evaluation of Cleaning Ability and Antimicrobial Action of New Herbal Ethanolic Mixture Extract as An Irrigant

Abstract

The aim of the present study was to evaluate a new herbal ethanolic mixture extract composed of (Syzigium aromaticum (clove), Eucalyptus globulus, Cinnamomum zeylanicum and Mentha piperita) as a root canal irrigant regarding its cleaning ability which was evaluated by using stereomicroscope and its antimicrobial action which was evaluated using a direct count of colony forming units (CFUs) and comparing it to the traditional root canal irrigants used, NaOCL and CHX. A total of eighty recently extracted human permanent fully formed single-rooted teeth were selected for this study, all samples were decoronated with a diamond wheel disc and the length is standardized to 15 ±1mm, then divided into two main groups. All samples were sterilized before and after de-coronation in an autoclave with pressure (15Psi) for 15 min at 121ºC. The four herbals were grounded to fine powder (50 mg) using spices grinder, pre-weighed on an electronic scale and soaked for ten days in 500ml of 95 % Ethyl alcohol, then filtered using filter paper. The final irrigant was prepared by mixing equal amounts from each extract, stored in dark bottle and kept refrigerated until use. The first part of the study was Evaluation of the Cleaning Ability of the New herbal mixture, 30 samples were used, randomly divided into Three experimental groups (10 each) according to the type of irrigant used during root canal instrumentation as follows, Group 1 (New Herbal Mixture), Group 2 (Sodium Hypochlorite 2.6%), Group 3 (Chlorohexidine 2%) Root canal instrumentation was done in a crown down technique using E-cube endodontic motor and M-pro rotary file system according to the manufacturer’s instructions (torque=300,150,150 gcm successively with a constant speed of 500 rpm) as follows, stainless steel K file #15 was used to check patency of the canals, Working length was determined once K file #15 appeared from anatomical foramen of root canal, Final WL (-1), Entire root canal was instrumented in a crown down technique by the endodontic motor using the file system with the help of 17%EDTA gel as a lubricant in the following operating sequence, a glide path created by a size #15 k file, then canal irrigation by using 3ml of the corresponding