CONSTRUCTION OF HIGH DENSITY GENETIC MAP FOR BREAD WHEAT THROUGH GENOME WIDE ASSOCIATION ANALYSIS

Abstract

The objectives of this study are to investigate the population structure of 70 Egyptian, Syrain and Iranian wheat accessions and to identify some markers associated with salinity tolerance in bread wheat. In addition to identify some genes that control some important agronomic parameters of wheat under salinity stress. The wheat germplasm panel was consisted of 70 accessions obtained from Egypt, Syria and Iran. The assessment of salinity tolerance was conducted over the years of 2018 and 2019 in the green house of Agricultural genetic Engineering research institute (AGERI), Agricultural Research Institute (ARC), and the experimental filed of faculty of environmental agricultural sciences, Suez university in Arish , Sinai, Egypt. This subset was chosen from the International Center for Agricultural Research in the Dry Areas (ICARDA) and Agricultural Research Center gene banks, Giza, Egypt. The genome association analysis (GWAS) and population structure analysis was conducted using six SCoT, five SSR and 93 SNP markers. A total number of 61 PCR-bands were revealed using SSR and SCoT primers, where SCoT analysis provided a higher number of bands (46 bands) compared to SSR analysis (15 bands). The maximum number of bands was obtained from SCoT-05 primer (10 bands). Additionally, the total number of polymorphic bands was 48 bands, where SCoT-10, and SCoT-01