Assessment of the effects of some commercial antiaging supplements on aging biomarkers in liver of male rats

Abstract

Aging is a multi-factorial process of biological and molecular alterations. The current research was undertaken to investigate the anti-aging efficacy of Astragalus membranaceus telomerase activator-65 (Ta-65) and pomegranate supplements on aging-induced liver apoptosis, male infertility, and kidney dysfunction. Male Wistar rats were divided into young rats, aged rats, aged rats+Ta-65 (500mg/kg/day), and aged rats+pomegranate (250mg/kg/day). Liver function tests, protein carbonyl, insulin-like growth factor-1 (IGF-1), testosterone, FSH, LH, and kidney function tests were measured in serum. Liver malondialdehyde, glutathione S-transferase, the expression of telomerase reverse transcriptase (tert), thioredoxin reductase1 (trxR1), and cytochrome 3a1 (cyp3a1) genes, in addition to hepatic p53 protein expression and capase-3 activity were evaluated. Sperm analysis as well as testicular histological