Scanning Electron Microscopic Observations On Collagen Fibers in Human Dentin

Abstract

The present study was designed to elucidate the. collagen fibrillar network of normal human coronal and radicular dentin. Thirty sound human teeth with closed apical foramina, from patients aged 15-25 yrs. were used in this study. The specimens comprised human premolars extracted as part of orthodontic treatment and impacted third molar teeth. The extracted teeth were then prepared for both light and scanning electron microscopy to examine the collagen fibers of dentin in the following groups : 0 Group (1) : Coronal dentin i) Under the cusp tip. ii) At the ceNical region of the crown. 0 Group (2) : Radicular dentin i)CeNical half of the root . ii)Apical half of the root . After extraction, 15 teeth were immediately fixed in formal calcium for 48-72 hours, then the teeth were demineralized in 5% EDTA and processed for paraffin embedding. Sections of 6um thickness were cut and stained with: 1- Hematoxylin and Eosin to demonstrate the normal histological feature of dentin.