Dysbiosis Induce Autoimmune Liver Injury: Study on the Effect of Indomethacin on the Intestinal Permeability in Juvenile versus Eldery Wistar Rats

Abstract

iver is a major organ doing many functions and consider one of immunological organs. The gut microbiota play an important role in the course of liver disease. Gut microbes target the liver due to the anatomical and functional connection between them. In healthy state there is a balanced interactions among bacteria, epithelium and gut immune system limit the access of pathogen and bacteria to the portal circulation and the liver. The intestine constitutes a reservoir of foreign antigens that can interact with mucosal immune cells and influence systemic immune responses. The dysregulation of intestinal immunity enhance the cross of food antigens and bacterial antigens with strong immune-activating properties through the epithelium which may lead to chronic immune-mediated inflammatory liver disease as autoimmune hepatitis (AIH). AIH is highly affected by gut microbiota as leaky gut and microbiome (dysbiosis) are present in patients with AIH and correlate with the severity of disease. Here in we used indomethacin to induce small-intestinal injury by alterations in the structure of the intestinal microbiota and disturb the intestinal permeability. Four groups (10 rats each) of male Wistar rats were divided into 2 main groups, adult group and juvenile group each is subdivided into control and indomethacin group (3mg/kg/day).