GENOTOXIC EFFECT OF MONOSODIUM GLUTAMATE (MSG) ON THE BONE MARROW CHROMOSOMES AND DNA AND STUDYING THE PROTECTIVE ROLE OF BEE PROPOLIS ON THE MALE ALBINO MICE

Abstract

Monosodium glutamate (MSG) is a commonly used flavor enhancing food additive derived from glutamic acid and used in many packaged foods, fast foods and restaurant foods also chips and noodles. Propolis is a natural substance produced by honeybees to protect its hive. So, it is antibacterial, antivirulence and antifungal also it is antioxidant, anti-inflammatory and scavenging free radicals. The present investigation deals with the genotoxic effects of monosodium glutamate and protective role of propolis on bone marrow chromosomes, DNA content and histological structure of liver of male albino mice Mus musculus. A total number of sixty CD-1 male mice (16-18 weeks old and 25-30 g) were used. They were divided into six main groups. The first group and 6th group contain 6 mice and other groups contain 12 mice. First group served as control while the 6th group received propolis (100 mg/kg b.wt.) only. The 2nd group and the 3rd group treated with 0.5 g/kg b.wt. and 1 g/kg b.wt. of monosodium glutamate respectively. The 4th group and the 5th group treated with 0.5 g/kg b.wt and 1 g/kg b.wt. of MSG in addition to propolis (100 mg/kg b.wt.) respectively. Each MSG and propolis treated animals were orally injected daily for 2 and 4 weeks with the selected doses.