Some studies on brucellosis in sheep and goats in Gulf region

Abstract

This study was performed to determine the sero-prevalence of sheep and goats (small ruminants) brucellosis in some areas of Arabian Gulf, using serological methods Rose Bengal Plate test (RBPT), indirect enzyme-linked immunosorbent assay (i-ELISA) and complement fixation test (CFT) in addition to using polymerase chain reaction (PCR) for blood and confirmed by mediaculture and PCR for tissue samples from positive reactors. The serological examination using RBPT revealed that the Infection rate was total (855/20067 (4.26%)) that rate in area 1 (62/8500 (0.729%)), area 2 (365/4977 (7.3%)), while in area 3 (428/6590 (6.49%), was animals were sero-positive. Using i-ELISA for confirmation cleared that out of the 855 RBPT-positive reactors, the i-ELISA were positively confirmed in 717/20067 (3.57%) animals, of which rate in area 1 (41/8500 (0.48%)), area 2 (293/4977 (5.89%)), while in area 3 (383/6590 (5.81%), were confirmed as positive. Using CFT for confirmation cleared that out of the 855 RBPT-positive reactors, the CFT were positively confirmed in 595/20067 (2.97%) animals, of which rate in area 1 (38/8500 (0.45%)), area 2 (246/4977 (4.94%)), while in area 3 (311/6590 (2.97%), were confirmed as positive. Using PCR blood for confirmation cleared that out of the 855 RBPT-positive reactors, the PCR blood were positively confirmed in 264/20067 (1.32%) animals, of which rate in area 1 (18/8500 (0.21%)), area 2 (118/4977 (2.37%)), while in area 3 (128/6590 (1.94%), were confirmed as positive.