Role of Gene –Xpert in diagnosis of Smear Negative Pulmonary Tuberculosis

Abstract

The health problem of TB infection was global with specific concern in developing country, the rate of death may reach to more than 1 million per year, thus input a major challenge in front of microbiologists to find accurate and rapid method for early detection of TB cases, for rapid treatment interaction (Parsons et al., 2011; Urdea et al., 2006). The usual methods that used in diagnosing TB cases was direct smear analysis under microscope after sating the bacteria by acid fast (AFB); the sensitivity of that methods was 50% (Young et al., 2008). The patients with negative smear may add a major problem to confirm the diagnosis; hence, the culture on LJ media was the gold standard method with duration time about 6 week. The longer duration that needed for beginning the management increased the burden of disease, so, the finding of rapid and accurate methods was mandatory to enhance the decision of proper treatment (WHO, 2016).