Biochemical Studies on Microbial L-Glutaminase And Its Applications

Abstract

The present study aimed to produce microbial extracellular L-glutaminase enzyme and using this enzyme as a powerful antioxidant and anticancer agent. Twelve recommended microbial strains were screened for the production of extracellular L-glutaminase enzyme, namely (seven fungi and five bacteria). The bacterial strain Escherichia coli ATCC 8739 was obtained from Microbiological Resources Centre (Cairo Mircen), Faculty of Agriculture, Ain Shams University, Egypt. While, Bacillus cereus NRC-20, Aspergillus terreus NRC-7, Aspergillus oryzae NRC-34 and Penicillium janthinellum Biourge are local strains obtained from the Culture Collection of the Department of the Natural and Microbial Products Chemistry, National Research Centre, Dokki, Egypt. In addition, Bacillus subtilis NRRL 1315, Trichoderma reesei NRRL 11460 and Trichoderma viride NRRL 6418 were obtained from the National Center for Agricultural Utilization Research, Peoria, Illinois 61604, U.S.A. Other four strains Pseudomonas aeruginosa ASU-1, Staphylococcus aureus, Aspergillus niger ASU-a6 and Penicillium cyclopium were local isolated strains provided from Microbiology Department, Faculty of Science, Ain Shams University.