Advanced Studies on Pasteurellosis in Farm Animals
Amany Dieb Bahr Dieb;
Abstract
The study was performed to achieve the following:
1. Clarification of some of the epidemiological aspects associated with P. multocida and M. haemolytica infections in the examined Egyptian cattle, buffaloes, sheep, and goats at Giza governorate.
2. Recording different clinical manifestations caused by P. multocida and M. haemolytica infections in the examined animals.
3. Laboratory diagnosis of P. multocida and M. haemolytica infections using bacteriological isolation, identification, and biochemical characterization.
4. Molecular identification of P. multocida and M. haemolytica isolates using polymerase chain reaction.
5. Molecular capsular serogrouping of isolated P. multocida using multiplex polymerase chain reaction.
6. Phenotypic detection of antimicrobial resistance of P. multocida and M. haemolytica isolates against different antimicrobials using disc diffusion method with the identification of multi-resistant strains.
7. Evaluation of the efficacy of a polyvalent killed vaccine prepared from locally isolated P. multocida and M. haemolytica strains and adjuvanted with MontanideTM ISA 71 VG oil for the prevention of experimentally induced infection in mice.
8. Biochemical and immunological evaluation of the prepared vaccine in sheep.
The results revealed that:
A total of 155 deep nasal swabs were collected from 20 cattle, 37 buffaloes, 80 sheep, and 18 goats of different ages and sexes in two seasons (winter and summer) from different farms located in the Giza Governorate.
Samples were collected from animals showing clinical signs of coughing, copious nasal and ocular discharge in association with rectal temperatures above 39.5°C, dullness, depression, congested mucous membranes, and respiratory discomfort.
1. Clarification of some of the epidemiological aspects associated with P. multocida and M. haemolytica infections in the examined Egyptian cattle, buffaloes, sheep, and goats at Giza governorate.
2. Recording different clinical manifestations caused by P. multocida and M. haemolytica infections in the examined animals.
3. Laboratory diagnosis of P. multocida and M. haemolytica infections using bacteriological isolation, identification, and biochemical characterization.
4. Molecular identification of P. multocida and M. haemolytica isolates using polymerase chain reaction.
5. Molecular capsular serogrouping of isolated P. multocida using multiplex polymerase chain reaction.
6. Phenotypic detection of antimicrobial resistance of P. multocida and M. haemolytica isolates against different antimicrobials using disc diffusion method with the identification of multi-resistant strains.
7. Evaluation of the efficacy of a polyvalent killed vaccine prepared from locally isolated P. multocida and M. haemolytica strains and adjuvanted with MontanideTM ISA 71 VG oil for the prevention of experimentally induced infection in mice.
8. Biochemical and immunological evaluation of the prepared vaccine in sheep.
The results revealed that:
A total of 155 deep nasal swabs were collected from 20 cattle, 37 buffaloes, 80 sheep, and 18 goats of different ages and sexes in two seasons (winter and summer) from different farms located in the Giza Governorate.
Samples were collected from animals showing clinical signs of coughing, copious nasal and ocular discharge in association with rectal temperatures above 39.5°C, dullness, depression, congested mucous membranes, and respiratory discomfort.
Other data
| Title | Advanced Studies on Pasteurellosis in Farm Animals | Other Titles | دراسات متقدمة عن عدوي الباستريلا في حيوانات المزرعة | Authors | Amany Dieb Bahr Dieb | Issue Date | 2021 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB7757.pdf | 694.77 kB | Adobe PDF | View/Open |
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