Study of long non-coding RNA and mitochondrial dysfunction in diabetic rats

Abstract

iabetes mellitus has become one of the major healthcare problems worldwide. Cardiovascular disease is a major cause of mortality in DM type 1 (T1DM) (Fan et al., 2019). Mitochondria are dynamic organelles that move, fuse, divide, and die. Mitochondria undergo fission and fusion to form dynamic interconnecting networks. The fusion and fission processes occur in a constant and balanced manner to adapt the mitochondrial network morphology to the metabolic needs of the cell (Duraisamy et al., 2019). The lncRNAs are implicated in development and progression of a number of different diseases. The H19 gene produces a non-coding RNA. The lncRNA H19 acts as miRNA precursor as exon 1 of H19 encodes two conserved miRNAs: miR-675-3p and miR-675-5p. Another function is to act as microRNA ‘sponge’ through its miRNA binding sites (Han et al., 2016). The current study was carried out at Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University (2018-2020) in collaboration with physiology department, Faculty of Medicine, Ain Shams University. The study was approved by Research Ethics Committee (REC),