ENVIRONMENTAL RISK FACTORS ON TELOMERE LENGTH MEASUREMENT OF ALZHELMER'S PATIENTS

Abstract

Background: Alzheimer’s disease (AD) is genetic disorder disease. Regulation of Telomere Length (TL) is a result of complex interaction between environmental and genetic factors. Aim: Compare and evaluate between TL measurement by Florescence In Situ Hybridization (Q FISH) and Real Time- PCR .Study of some environmental risk factors in the development in AD. Subjects and Methods: This study included 30 AD patients and 10 controls. All blood samples were collected measured by using Q-FISH and RT-PCR to assess the telomere length. Results: Our data showed a significant difference in TL between AD patients and the controls P = 0.036. The correlation between results of Ratio of telomere repeat copy number to single copy gene copy number (T/S) ratio by RT-PCR and TL (Kb) by (Q-FISH) by linear regression analysis the correlation coefficient, r2 , for the relationship of T/S ratio to TL (KB) was =0.266 and P=0.004. Conclusion: A significant correlation was found between AD and telomere length shortening. Some environmental factors such as diabetes and hypertension influences on TL in AD. RT PCR is more accurate and easy than Q FISH.