SYNTHESIS OF STRUCTURED LIPID AND IMPROVING ITS OXIDATIVE STABILITY USING POTENT ANTIOXIDANT EXTRACTED FROM OLIVE LEAVES

Abstract

Synthesis Of Structured Lipid and Improving Its Oxidative Stability Using Potent Antioxi¬dant Extracted from Olive Leaves

The objectives of this work are divided into four objects. First target is to synthesis feruloylated structured lipids, through biocatalytic in¬corporation of ferulic acid into vegetable oil lattice in solvent free system (vegetable oil as reaction media) using individual and combinational lipase systems of three commercial lipases; two unspecified lipases, NovoCor ADL and Novozyme435®; and one 1,3-specified lipase, Lipozyme TL 100L. Second object is the extraction of potent antioxidants from olive leaves using aqueous sodium bisulphate solution at 45°C with different pH values and different extraction time followed by the determination of major phenolics such as oleuropein. Third object is the determination of the antiradical capacity of the formed FSLs and enhance its oxidative stabil¬ity via adding the olive leaves extract in ethyl acetate media. Fourth object, present the cheap lipases TLL and ADL as suitable alternative option of expensive N435 for industrial scale application and forming nutritive lipid. Three types of vegetable oils were selected according to their highest fatty acid content, health benefits, and their uses in food industry. Linseed oil is one of the richest oils of the omega-3 fatty acid, α-linolenic acid (ALA). Also, it possesses bioactive properties useful for health benefits. However, it is very susceptible for oxidation Canola oil is omega-6 rich oil, linoleic acid (LA), useful for health and its wide use in foods. Palm stearin is one of palm oil fraction contains 60 % of palmitic acid; that has massive use in food industry in forms of shortening, margarine, and other forms. Lipozyme TL 100L and NovoCor ADL considered as two novel use low-cost lipases in feruloylated structured lipids in comparison to wide used Novo-zyme435® lipase. Ferulic acid possesses high free radical scavenging capacity, excellent UV-absorbing, and antitumor activity. However, ferulic acid has poor solubility in nonpolar media. Therefore, the incorporation will be beneficial for both ferulic acid by increasing its lipophilization and oils by obtaining multifunctional properties combining, for instance, rising their oxidative stability combined with nutritive value for food and cosmetic industries us-es.