EFFECT OF GAMMA IRRADIATION ON SOME SELECTED ISOLATES OF MICROALGAE FOR OPTIMIZING BIODIESEL PRODUCTION
MARWA MOHAMMED MOHAMMED MOUSSA;
Abstract
The present study was designed to investigate the effect of different conditions on the growth, total lipid yield, and the composition of extracted FAMEs of three green microalgal species; Chlamydomonas sp., Scenedesmus ecornis, and S. communis, in order to determine the best conditions for subsequent biodiesel production.
To accomplish this goal, various techniques of cell disruption and some organic solvents were tested for their effect on extracting the highest yield of total lipids as well as the best proportions of palmitic and oleic fatty acids from each microalga. Box˗Behnken model was then applied to identify the optimum level of each variable; involved both nutritional and environmental factors, which suit every microalga. After that, each microalga was exposed to stress conditions (gamma irradiation and macronutrient depletion) were explored for their impact on its growth and lipids productivity. The microalgal co˗culture was applied to enhance the productivity of biomass and total lipids and to track its impact on the extracted FAMEs composition from mixed biomass.
The most significant findings could be summarized as below:
1. The osmotic shock was shown as the best disruption procedure that boosted the efficiency of lipid extraction from wet microalgal biomass, by releasing the highest yields of lipids and total FAMEs; involving SFAs and USFAs ratios. Results indicated the following:
The total lipids yield and total FAMEs extracted from Chlamydomonas sp. reached a maximum of 25.1 and 17.7 % (w/w), respectively. The highest percentages of SFAs and USFAs were observed at 24.1 and 39.3 % of total FAMEs (w/w), to reach 1.5˗ and 2.0˗fold of control levels, respectively.
For S. ecornis, the extracted total lipids and total FAME yields reached up to 28.3 and 23.7 % (w/w), respectively. Furthermore, the increased proportions of total SFAs and USFAs were recorded to reach 25.5 and 37.8 % of total FAMEs (w/w), which equal 1.4˗ and 1.6˗fold of control values, respectively.
To accomplish this goal, various techniques of cell disruption and some organic solvents were tested for their effect on extracting the highest yield of total lipids as well as the best proportions of palmitic and oleic fatty acids from each microalga. Box˗Behnken model was then applied to identify the optimum level of each variable; involved both nutritional and environmental factors, which suit every microalga. After that, each microalga was exposed to stress conditions (gamma irradiation and macronutrient depletion) were explored for their impact on its growth and lipids productivity. The microalgal co˗culture was applied to enhance the productivity of biomass and total lipids and to track its impact on the extracted FAMEs composition from mixed biomass.
The most significant findings could be summarized as below:
1. The osmotic shock was shown as the best disruption procedure that boosted the efficiency of lipid extraction from wet microalgal biomass, by releasing the highest yields of lipids and total FAMEs; involving SFAs and USFAs ratios. Results indicated the following:
The total lipids yield and total FAMEs extracted from Chlamydomonas sp. reached a maximum of 25.1 and 17.7 % (w/w), respectively. The highest percentages of SFAs and USFAs were observed at 24.1 and 39.3 % of total FAMEs (w/w), to reach 1.5˗ and 2.0˗fold of control levels, respectively.
For S. ecornis, the extracted total lipids and total FAME yields reached up to 28.3 and 23.7 % (w/w), respectively. Furthermore, the increased proportions of total SFAs and USFAs were recorded to reach 25.5 and 37.8 % of total FAMEs (w/w), which equal 1.4˗ and 1.6˗fold of control values, respectively.
Other data
| Title | EFFECT OF GAMMA IRRADIATION ON SOME SELECTED ISOLATES OF MICROALGAE FOR OPTIMIZING BIODIESEL PRODUCTION | Other Titles | تأثير التشعيع الجامي على بعض العزلات المختارة من الطحالب الدقيقة لتعظيم انتاج الديزل الحيوي | Authors | MARWA MOHAMMED MOHAMMED MOUSSA | Issue Date | 2021 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB8874.pdf | 1.05 MB | Adobe PDF | View/Open |
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