Biocontrol of some Pathogenic Microorganisms Using Milk Whey, Moringa plant and Nanoparticles.

Abstract

The fast growing of microorganisms resistant to multi antimicrobial drugs have vast impact on mortality and human healthcare. The antimicrobial peptides are highly active against most microbes such as Gram-positive and Gram-negative bacteria, fungi, viruses by disruption of cell membrane. The plants have been used for medicinal purpose as old as the history of mankind. Thus extraction and characterization of several active phytocompounds from the medicinal plants is the basis for the formation of some high activity profile drugs. Further, the phytochemical contents and pharmacological actions. Moringa plants having medicinal potential remain UN assessed by rigorous scientific research to define efficacy and safety. The leaves, fruits, flowers and immature pods of this tree are used as a highly nutritive vegetable in many countries. M. oleifera leaves has been reported to be a rich source of -carotene, protein, vitamin C, calcium and potassium and act as a good source of natural antioxidants. 1) one hundred isolates collected from different patients suffered from different diseases from ZUH, and from these isolates only 32 isolates showed blood hemolysis, these 32 isolates experimented against buffalo whey protein and moringa leaves and seeds extracts and the most sensitive isolates identified by biochemical testes and molecular identification by 16SrRNA which identified as Staph. pasteuri MN368257. 2) In this study buffalo whey protein was hydrolyzed with papain, trypsin and pepsin enzymes to liberate the antibacterial peptides. The extent of protein degradation by trypsin was estimated by assessing the degree of hydrolysis (DH) and SDS-PAGE analysis. The hydrolysate obtained after 180 min. degradation had the