The possible protective effect of Resveratrol on Naphthalene induced nephrotoxicity in Adult Male Albino Rat

Abstract

ecent clinical observational studies claimed that the use of Resveratrol might have a protective effect against a wide variety of nephrotoxic agents that might progress to end-stage renal diseases. The aim of the work was to verify these claims by an in vivo experimental study in the adult male albino rat. Thirty-six adult male albino rats were assigned into three groups: a control group (I) in which rats were not administrated for any treatment. In-group (II), rats received daily oral naphthalene in dose of 200 mg per kg dissolved in 5 mg per kg corn oil for 4 weeks successively. In group (III), rats received resveratrol orally dissolved in propylene glycol by gastric tube at a dose 10mg per kg followed after 60min by naphthalene at a dose 200mg per kg dissolved in 5ml per kg of corn oil daily for four weeks. Then animals were sacrificed, and kidney specimens were processed into paraffin blocks, sectioned, and stained with H&E, Masson’s trichrome & PAS. Stained sections and image analysis were used to count vacuolated tubular cells, tubular cells with pyknotic nuclei, tubular casts and glomeruli with thickened glomerular basement membrane, then data was subjected to statistical analysis