The potential role of mesenchymal stem cells derived exosomes on the healing of grade II experimentally induced skeletal muscle injury in adult male albino rats. Histological study

Abstract

Skeletal muscle injury is the most common cause of severe long-term pain and physical disability, especially in athletes. Failure of proper treatment may postpone the return of an athlete to sport for weeks or even months. The most frequent complication of muscle injury is the recurrence, which occurs due to weakness of injured muscle and presence of residual scar tissue. The fibrotic scar reduces the contractile capacity of the muscle. Recently, the use of exosomes is an emerging cell-free therapy that promote tissue repair and regeneration without the risks associated with direct stem cell transplantation. The aim of this work was to study the potential role of MSCs- derived exosomes on the healing of experimentally induced grade II skeletal muscle injury in adult male albino rats. Forty male albino Wister rats were used in this study. Five out of the forty were young weaned male albino rats that were used for isolation of exosomes. Isolation of exosomes was done by ultracentrifugation method from bone marrow derived-MSCs then characterization was done using transmission electron microscope. The remaining 35 rats were adult and randomly divided into four groups. Group I (n=10): served as control and were subjected to Sham operation in which all steps of grade II muscle injury surgery were done without incisional muscle biopsy. Group II (n=10): served as untreated muscle injury group. They were subjected to grade II m