The Effect of Low Level Laser on Enzymes Involved in DNA Repair

Abstract

This Study aims to investigate the capability of activation of DNA repair enzymes involved in the biological cell that hosted a DNA damage via the exposure to Low Level Laser (LLL) at different fluences. The need for DNA repair enzymes activation or activation of DNA repair process becomes a serious demand after increasing genotoxic agents’ sources and diseases caused by DNA damages. Low Level Laser (LLL) has been reported as an effective therapeutic tool in pain relief, wound healing, deeper tissues and nerves healing and bone regeneration. In addition, it could produce protection effect against DNA damages subsequent to ionizing radiation exposure and the proliferation in the biological cell. In this study, low level He-Ne laser was supposed as activator to the DNA repair process or DNA repair enzymes in human peripheral blood lymphocytes. These lymphocytes had DNA damages due to the previous exposure to different low doses of γ-radiation (2, 4, 6, 8, 10) Gy in Cs-137 unit. The effect of LLL was observed at different fluences (1, 2.5, 5) J/cm2. Comet assay was used here to monitor the capability of LLL in removing the DNA damages produced by gamma radiation in the lymphocytes.