Effect of Nigella sativa on silver nanoparticles induced hepato-nephrotoxicity in Clarias gariepinus

Abstract

The present work has been planned to investigate the effect of Ag-NPs on selected biochemical parameters of serum, liver tissue and kidney tissue in Clarias gariepinus. This study was carried out also to evaluate the effect of Ag-NPs on the oxidative stress, antioxidant enzyme and DNA fragmentation. In addition, this study aimed to highlight the histopathological change to Ag-NPs exposure. Finally, to represent the role of NS in the improvement of these parameters. Experimental design and investigated parameters: In the present study 168 catfish (C. gariepinus) were used. 48 fish were used to estimate the (LC50) of Ag-NPs. The other 120 fish were divided into four groups and each group consisted of 3 replicates of 10 fish per aquarium. Group I was served as control group. Group II served as control group and co- administrated with 3% NS. Group III was exposed to 50 mg/L Ag-NPs. Group IV was exposed to 50 mg/L Ag-NPs and co- administrated with 3% NS. After 30 day of treatment, blood samples were collected from the caudal blood vessels. Serum was collected, liver and kidney were rapidly excised for the biochemical and histological studies. The present work included the following investigations: - Clinical investigation and post mortem examination - Serum: Determination of serum levels of ALP, ALT, AST, urea creatinine, uric acid, total proteins, Albumin, Globulin, glucose, cortisol, LDH, cholesterol and triglycerides. - Liver and kidney determination of LPO, GSH, CAT, SOD and TAC content. - DNA fragmentation analysis. - Liver and kidney histopathological changes. The results: The half lethal concentration (LC50) of Ag-NPs was observed through exposure of catfish (C. gariepinus) was 171.7 mg/L in 96 h.