Molecular characterization of non-typhoidal Salmonella serovars isolated from human and animals In Egypt

Abstract

The present study was concerned with the isolation of NTS from febrile neutropenic Egyptian patients suffering from severe gastroenteritis, and also from animals of the same geographical area and during the same period, and screening them against different antimicrobials agents commonly used to treat salmonellosis. The aim was to investigate the etiology (phenotyping, genotyping) of infections by different NTS isolated from humans and animals, and to study the effect of different antibiotic combinations on some selected resistant isolates for the purpose of combating antimicrobial resistance. A total of 300 human fecal samples were collected from febrile neutropenic patients suffering from severe gastroenteritis, and were bacteriologically examined. About 8.66% of them were Salmonella of which 16.66% of the isolates were NTS, and 2% of the isolates were typhoidal Salmonella. Serological identification of the recovered Salmonella isolates showed that they belonged to 15 different serovars which were S. Typhimurium (17.85%), S. Enteritidis (14.28%), S. Lumberhurst (14.28%), S. Tumodi (7.14%), S. Tsevie (7.14%), S. Butontan (4.28%), S. Anatum (4.28%), S. Taksony (4.28%), S. Hull (4.28%), S. Agama (4.28%), S. Dublin (4.28%), S. Blegdam (4.28%), S. Paratyphi A (4.28%), S. Paratyphi C (3.57%), S. Paratyphi B (1.78%). Also, a total of 50 serologically identified NTS serovars recovered from animals of the same geographical area and during the same period as well as one standard strain S. Poona were supplied by the Bacterial Bank of AHRI of Egypt. Antibiogram analysis revealed that more than 90% of both human and animal serovars were resistant to clindamycin, erythromycin, rifampicin, amoxicillin, lincomycin, tobramycin, ampicillin, oxytetracycline, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefotaxime. On the other hand, more than 80% of both human and animal serovars were sensitive to imipenem, cefoperazone/sulbactam, colistin/sulfamethazine, followed by ciprofloxacin. While, the standard strain exhibited similar sensitivity pattern to the used antibiotics.