Effect of Different Natural Cross-linkers on the Durability of the Resin-Dentin bond and the Stiffness of Dentin: An in Vitro study

Abstract

The aim of this study was to evaluate the effect of different natural cross-linkers on the microtensile resin-dentin bond strength after 24 hours and 6 months of storage and to assess the durability of this bond as well as the effect of these extracts on the stiffness of demineralized dentin matrix. Specimens were prepared and divided according to the type of natural extract used into 5 groups; control, grape seed extract, cacao seed extract, sumac extract and curcumin extract. For the micro-tensile bond strength testing, the specimens of each group were tested after 24 hours and 6 months of water storage. For the stiffness test, the different experimental groups were tested after treatment with the extract for 1 minute. For the microtensile bond strength test, thirty recently extracted sound teeth were distributed on the 5 testing groups. The teeth had their occlusal enamel removed and exposed dentin was acid-etched and rinsed. The respective pre-treatment was then applied to the dentin surface for 1 minute and the adhesive resin was applied. Composite resin was built in 2 increments (2mm each). Teeth were then sectioned into 1x1x8mm beams and the test was performed using a universal testing machine in the tensile mode. Fracture mode analysis was performed using a stereomicroscope at 40X magnification. The bonded interfaces before and after storage were examined under a scanning electron microscope.