HISTOPATHOLOGICAL, HISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDIES ON ASEWAGE POLLUTED FRESH WATER FISH

Abstract

Nowdays, many farmers intended to use sewage water for irrigation of fish ponds, consequently accumulation of heavy metals .led to hazardous effects for fishes. So this work had been done to make spot light on the effect of sewage on the histology, histochemistry and electron microscopy of some fish organs; liver, kidney and gills of one of the most famous cultivated fish (the common carp, Cyprinus carpio). The fingerlings were obtained in one lot alive from Abbasa-Fish hatchery Sharkia provience. They are housed in glass aquaria (of 100 liter in size) under the laboratory conditions, including natural photoperiod and temperature (20-25 C). Two glass aquaria were adjusted and prepared, as to the first one raw sewage from Bahr El-Bakar drainage was added slowely up to the concentration become 25%. Whereas fishes in the second aquarium were left in fresh water as a control group. To each aquarium 50 healthy carp fries, of 2-3 cm, were transferred. At the same time and under the same laboratory conditions, four other aquaria filled with natural fresh water and 200 fish specimens were housed in each. To the first three ones, a standered solutions of Hg Cl2, Cd CI and Pb CI of pure grades, were added gradually till the concentration become almost 1.0; 4.0 and 7.0 ppm, respectively. Whereas, the fourth aquarium was left as a control. All the test solutions were renewed each 24hr.