“Vertical Ridge Augmentation Using Perforated Barrier Membranes and (L-PRF) Covered Xenogeneic Block Grafts – Experimental Study in Dogs”

Abstract

This experimental study was conducted in a total of six adult mongrel dogs each weighing a minimum of 20 kg and exhibiting a fully erupted permanent dentition. During the experiment, the dogs were fed once per day with a soft food diet. All the protocol procedures were revised and approved by the research ethics committee of the faculty of dentistry, Ain Shams University.

The experimental aspect was performed in three surgical phases. In the first phase, the mandibular 2nd, 3rd and 4th premolar (P2–P4) were extracted. A total of four standardized saddle-type defects (mesiodistal width: 10mm; height: 8mm) were bilaterally prepared in the lower jaw of each dog (i.e. n=4 defects per animal in the lower jaw). Following a healing period of 2 months, a xenogeneic block graft was utilized in all groups. (second phase).

In all groups, the xenogeneic block graft was fixed by osteosynthesis screw 12 or 14 mm in length and 1.5 mm in width with a cross flat head. All attempts were made so that the screw head was at the same level as the neighbouring bone crest. The lines of demarcation between the block and pristine bone and all voids were grafted by xenogeneic cancellous particles. In all surgical sites, tension-free primary soft tissue closure was achieved through periosteal releasing incisions, followed by horizontal mattress and interrupted sutures