Efficacy of Umbilical Cord Blood-derived Stem Cells Transplantation versus their conditioned media in Hypothyroid Rats

Abstract

he promise of regenerative medicine lies in the ability to produce tissues to replace damaged or diseased tissues. The use of stem cells for both autologous and heterologous transplants might cause ectopic growth, tumorigenesis, and immune system reactions. By contrast, the adoption of a cell-free therapy would significantly reduce the risks and overcome the difficulties associated with direct cell injection. The stem cell secretome, in addition to avoiding cell therapy side effects, has substantial advantages in manufacture, storage, and standardization, making it a promising type of biopharmaceutical. Even though an exhaustive characterization of the components of the conditioned culture medium, which also includes the microvesicles fraction, must be done, this emerging therapeutic treatment is a very encouraging, innovative therapeutic approach for regenerative medicine Owing to their proangiogenic potential, which is mainly mediated by paracrine factors, they are a promising treatment strategy for diseases. But instead of transplanting autologous or allogeneic MSCs, a new option is cell-free therapy, where MSCs are first cultivated and their EVs are then isolated and further manipulated to achieve a more proangiogenic cargo.