THE INFLUENCE OF GROWTH FACTORS ON THE MICROPROPAGATION OF STRAWBERRY PLANTS

Abstract

The present investigation was mainly conducted to study the effect of light intensity on culture survival percentage, the effect of some plant growth regulators on the Fragraia x ananassa cv. Festival micropropagation, anatomical structure, chemical, and growth parameters. Series of experiments were carried out successively during the years 2016 to 2019. The highest positive light intensity effect on survival percentage (100%) was observed under dark conditions. BA treatments on runner tips led to increment in total shoots and the concentration of 0.5 mg l-1 BA was the best. Also, concentration 0.5 mg l-1 BA recorded the highest value in both of chl a, and chl b. All TDZ treatments resulted in adventitious shoots development on leaf explants without callus formation. Growth in 1.5 mg l-1 TDZ led to an increment in the most morphological parameters compared to the rest treatments. The results showed that 1.5 mg l-1 of TDZ treatment significantly increase the number of shootlets per explant (5.0), total shoot (51.3), and the number of leaves/explant (12) as compared with other treatments. Accumulation of chlorophylls, total sugars, and total soluble phenols was enhanced by 1.5 mg l-1 of TDZ. This procedure provides a simple and rapid approach to regenerate strawberry plants via direct organogenesis.