Extraction, Isolation and Identification of some Bioactive organic Compounds from Opuntia ficus-indica (L.) Mill and Diospyros kaki

Abstract

This thesis aims to identify the chemical components, especially polyphenols, from two locally grown Egyptian plants, namely, the flowers of Opuntia ficus- indica which belongs to the family Cactaceae and the leaves of Diospyros kaki which belongs to the family Ebenacea and to study their applications against and anti- parasitic activities. The plants have been selected to be the subject of this thesis due to its economic and pharmacological importance and the lack of literature concerning their constituents specially the selected part in the current literature. The thesis contains two main parts. Part I: includes

1- The literature review part which comprises detailed account of the previously isolated natural compounds. 2- Methods and materials for isolation of flavonoids.

3- Phytochemical investigation of the active extracts of Opuntia ficus-indica flowers and Diospyros kaki leaves.

The methanolic extract of Opuntia ficus-indica flowers were subjected to Sephadex LH-20 column chromatography. Gradient