Antigenic and genetic mutations of rotaviruses in sheep and goats

Abstract

'On this study we were able to reveal the presence of rotaviruses that infects sheep and goat population in Egypt. These viruses were isolated from

fecal samples that were collected from neoborn diarrheic and clinically normal lambs and goat kids in 7 different grouping localities in Egypt. Using LAT and ELISA based on the use of VP6 monoclonal antibodies, it was possible to detect RV in 13.4% and 3.2% in samples from lamb and goat kids respectively by the LAT. Whereas by Mabs ELISA we could detect it in 38.8% and 57.8% from lamb and goat kids respectively.

Comparing the ability of both tests todetect the presence of RV in the fecal samples, the ELISA superseded and was able to detect a total of 50 more positive samples with a 34% than the LAT. In a trial to isolate these viruses from some highly positive samples by both LAT and ELISA, 8 samples were inoculated on MA-104 for 5 successive passages, the harvestsof such passages were tesed for the presence of the virus by ELISA basd on the use of Mabs. It was possible to detect the virus only in the harvests of the first passage, although it was not possible to detect it in any further passage. Whereas detecting RV in the original fecal samples using ELISA based on the use of Mabs directed to the outer capsid protein VP7, 3 positive fecal samples out of the 8 tested were detected. A genomic characterization of the RV in these 3 samples