Advanced studies on Toxoplasmosis in small ruminants with reproductive disorders

Abstract

Toxoplasmosis is an infectious disease caused by protozoan T. gondii which causes reproductive failure in small ruminants. Newly developed methods as Genotyping is essential to explain the virulence, epidemiology, and effective treatment and control in human and animals. This study was conducted to assess the seroprevalence of T. gondii in sheep and goats in Egypt especially those with a history of reproductive disorders including abortion and molecular identification of T. gondii followed by studying the genetic variety of T. gondii isolates rotating in Egypt. Blood samples were collected from 138 and 212 live ewes and she-goats from five Governorates of Egypt. Also, the blood and its corresponding tissue samples were collected From 180 ewes and 206 she-goats from (El-Moneib, El-Warak and El-Basatin abattoirs) at Cairo, Giza governorates of Egypt. All samples were serologically examined using ELISA and the tissue samples of the seropositive animals were digested and microscopically examined then bio-assayed in mice. All the T. gondii isolates were subjected to molecular identification using PCR and genotyped using nested PCR (n-PCR) combined