"Evaluation of Antibacterial Properties and Smear Layer Removal of Sage as a Root Canal Irrigant for Primary Anterior Teeth: An In-Vitro Study"

Abstract

One of the most important reasons of endodontic treatment failure is the persistence or survival of microorganisms in the complex root canal system or periapical area; therefore, the success of endodontic treatment depends, to a great degree, on the elimination of microorganisms from the root canal system through mechanical instrumentation and chemical irrigation. The purpose of this study was to compare the antibacterial effect and smear layer removal of 0.2% Sage extract with 2% Chlorhexidine and Saline when used as root canal irrigants in extracted primary teeth . Seventy five roots were used in the current study, 60 roots of them were used for antibacterial testing and 15 roots were used for smear layer removal testing (5 for each group), 5 roots were irrigated 0.2% Sage leaves extract, 5 roots were irrigated with 2% CHX and 5 roots were irrigated with Saline. For the antibacterial testing the 60 roots were divided into two experimental groups , one positive control group and one negative control group as follows: Group 1: consisted of 20 root that were contaminated with E.faecalis and irrigated with 0.2% Sage solution. Group 2: consisted of 20 root that were contaminated with E.faecalis and irrigated with 2% CHX di-gluconate. Group 3 (positive control) : consisted of 10 root that were contaminated with E.faecalis and irrigated with normal saline. Group 4 (negative control) : consisted of 10 roots that were not co