Characterization of Some Antibiotic Resistant Genes Isolated from Pathogenic Staphylococcus aureus

Abstract

The aim of this work is to investigate the presence of mecA gene and class 1 integron between multidrug resistant MRSA. In this study a total of two hundred and twenty staphylococcal isolates were collected from 3 Egyptian hospitals (Abbassia fever hospital, Cairo hospital and Ain-shams hospital). Clinical samples were collected from urine, pus discharge and wound, (54%) isolates were recovered from urine, (40.4%) from wound and (13.6%) from pus discharge, (45.5%) were from males and (54.5%) were from females. All of the isolates were identified as Staphylococcus aureus (SA) as they produce golden yellow colonies on mannitol salt agar medium, also black, shiny and convex colonies with clear zones on Baird-Parker agar media and they were positive for catalase, coagulase. In addition, isolates were confirmed as S. aureus by Microscan biotyper automated system. Antibiotic susceptibility test showed that the isolates were multidrug resistance against the tested antibiotics with various extents. The percentage of antibiotic resistance can be summarized as; 81.8% of isolates were resistant to methicillin, 65% were resistant to trimethoprim-sulphamethaxole, 60% were resistance to amikacin, 46.3% were resistance to ciprof