NEPHRO-PROTECTIVE EFFECTS OF GRAPE AND GUAVA SEEDS EXTRACT ON GENTAMICIN INDUCED NEPHROTOXICITY IN RATS

Abstract

The present study was undertaken to investigate the nephroprotective effect of grape and / or guava seeds extracts on gentamicin – induced nephrotoxicity in rats. Rats were divided into eight experimental groups. Group 1 (control group) was injected intraperitonealy with saline solution; Groups (2, 3 and 4 were administrated a daily oral dose of grape (40mg/kg/d p.o), guava seeds extracts (300mg/kg/d p.o) or mixture of both, respectively; to demonstrate their safety. Group 5 was intoxicated with gentamicin (100mg/kg/day i.p). Groups (6, 7 and 8) received oral dose of grape (40mg/kg/d p.o), and / or guava seeds extracts (300mg/kg/d p.o) along with intraperitonealy injection of gentamicin to investigate the beneficial effect of these extracts against gentamicin nephrotoxicity for a period of consecutive 10 days. At the end of treatment period, 10 days (24 hours after the last injection), and the animals were kept individually in metabolic cages. Body weight of rats, absolute and relative kidney weights were determined. The following parameters were measured: serum level of creatinine, urea and uric acid. Serum levels of total protein, sodium, potassium, activity of alkaline phosphatase and glutathione (GSH). Renal content of malondialdehyde (MDA), nuclear factor kappa (NF) glutathione (GSH) and kidney injury molecule-1 (KIM-1),