Bioremediation of cyanide compounds by using some transgenic micro algal species
Rasha Abed Abd–Elmonem Mohammed;
Abstract
Development of novel technologies for the remediation of industrial and hazardous wastes is increasing rapidly. Particular attention is being focused upon the usage of biological treatment systems. Aerobic and anaerobic microbial treatment processes have been successfully employed in the destruction and/or removal of organic compounds, inorganics and metals.
Cyanide is a known toxic chemical produced through anthropogenic activities and industries that use ore leaching, electroplating, steal production, plastics, and synthetic fibers.
In this study, the cyanobacterial cyanase enzyme was targeted to cytosol and overexpressed in Chlamydomonas reinhardtii via Agrobacterium tumefaciens mediated co-culivation transformation method in order to establish a synthetic pathway for cyanide degradation. Transgenic C. reinhardtii algae containing the necessary genes for the novel pathway were generated. Variable amounts of RNA were detected by qRT-PCR. Simultaneous over-expression of cyanase would ultimately result in the formation of CO2 and NH3 from cyanide.
Biochemical and physiological analyses were performed under cyanide stress conditions using C. reinhardtii transgenic for evaluating the impact of the novel pathway in algae:
A) CYN-overexpressor and wild type C. reinhardtii were allowed to grow under different concentrations of cyanide (25, 50, 100,150 and 200 mg/L). CYN transgenic strain showed the specific activity of cyanase enzyme, which was obviously more than their corresponding activities in wild species. The study demonstrated that the ability of Cr.CYN genetically modified strains to grow at high concentrations of cyanide compared to the unmodified strain, especially at 150 and 200 mg/L.
B) The study showed that cyanide had an inhibitory effect on mean growth rate, relative growth rate, generation time and number of recycling of both wild and transgenic samples as compared to the control.
C) To evaluate the percentage of removal of cyanide in both microalgae, transgenic type and wild type was treated with different concentration of cyanide (25, 50, 100, 150 and 200 mg/L), the modified Cr.CYN strains showed high cyanide removal
Cyanide is a known toxic chemical produced through anthropogenic activities and industries that use ore leaching, electroplating, steal production, plastics, and synthetic fibers.
In this study, the cyanobacterial cyanase enzyme was targeted to cytosol and overexpressed in Chlamydomonas reinhardtii via Agrobacterium tumefaciens mediated co-culivation transformation method in order to establish a synthetic pathway for cyanide degradation. Transgenic C. reinhardtii algae containing the necessary genes for the novel pathway were generated. Variable amounts of RNA were detected by qRT-PCR. Simultaneous over-expression of cyanase would ultimately result in the formation of CO2 and NH3 from cyanide.
Biochemical and physiological analyses were performed under cyanide stress conditions using C. reinhardtii transgenic for evaluating the impact of the novel pathway in algae:
A) CYN-overexpressor and wild type C. reinhardtii were allowed to grow under different concentrations of cyanide (25, 50, 100,150 and 200 mg/L). CYN transgenic strain showed the specific activity of cyanase enzyme, which was obviously more than their corresponding activities in wild species. The study demonstrated that the ability of Cr.CYN genetically modified strains to grow at high concentrations of cyanide compared to the unmodified strain, especially at 150 and 200 mg/L.
B) The study showed that cyanide had an inhibitory effect on mean growth rate, relative growth rate, generation time and number of recycling of both wild and transgenic samples as compared to the control.
C) To evaluate the percentage of removal of cyanide in both microalgae, transgenic type and wild type was treated with different concentration of cyanide (25, 50, 100, 150 and 200 mg/L), the modified Cr.CYN strains showed high cyanide removal
Other data
| Title | Bioremediation of cyanide compounds by using some transgenic micro algal species | Other Titles | المعالجة الحيوية لمركبات السيانيد بأستخدام بعض الطحالب الدقيقة المعدلة وراثياً | Authors | Rasha Abed Abd–Elmonem Mohammed | Issue Date | 2022 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| BB12016.pdf | 948.71 kB | Adobe PDF | View/Open |
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