Evaluation of Nano-Albumin Based Antibiotic Delivery for Treatment of Bacterial Infection in Experimental Animals

Abstract

Infectious diseases represent a great risk as more than half of the deaths happening worldwide. Brucellosis is one of the diseases that caused by Brucella species, a bacterial pathogen. Despite the profound success achieved by the usage of antibiotics against infectious diseases, bacterial infections still conseder one the greatest challenges the in global healthcare. In the last few years, nanotechnology allows the development of new products that are used in human medicine; for quick and specific drug delivery into cells and tissues. The present study was aimed at preparing and characterizeing the nano- albumin particles and evaluateing thier safety on living lymphocyte cell culture for their potential therapeutic as drug carrier to enhance the treatment of brucella infection in male Balb.c mice. The study was divided in to three parts, the first part aimed to prepare the nano-albumin particles using the desolvation technique. Three techniques were used for characterization of the prepared particles; Transmission Electron Microscopy (TEM) was used to determine the particles shape and size; Zeta Sizer to estimate the zeta potential of the surface of the prepared nano particles and the size of this particles and finally by using X Ray Diffraction (XRD) technique to assess the chemical structure of the prepared nanoparticles. The second pa