Immunomodulatory Effects of Certain Fungal Metabolites on Some Selected Cancer or Viral Infected Cell Lines

Abstract

The aim of the present study is to determine the potential and promising fungal metabolites that might has cytotoxic and immunomodulatory effect on cancer or viral infected cell followed by characterization and formulation of the extracted metabolites for the purpose of human consumption.Therefore, to achieve this aim, Collection of fungal isolates from different Egyptian localities was needed. Followed by, extraction of fungal metabolites from the collected fungal isolates.Evaluation of cytotoxic activities of the recovered metabolites against Peripheral Blood Mononuclear Cells (PBMCs). Evaluation of the immunomodulatory effects of the selected metabolites that showed lowest cytotoxicity, on some selected cancer or viral infected cells and characterization of the metabolites showing promising Immunomodulatory effects.

Humanity continues to suffer the scourge of cancer, a disease that is associated with uncontrolled cell growth. In 2013, it was reported to be among the leading causes of death, second to cardiovascular diseases. It is estimated that death due to cancer will rise to thirteen million in 2030. The search for natural anticancer products is needed.

Recorded results have demonstrated the bioactive components of different five edible mushroom species. P. ostreatus , P. columbinus, P. Sajor-caju , L. edodes and A. bisporus were identified by the ITS sequenceing and resulting consensus ITS sequences were blasted in the NCBI (http://www.ncbi.nlm.nih.gov) database with the BLASTn for homology in order to identify the probable mushroom in question. The sequences were deposited in the Gen-Bank® with accession numbers MK603976, MZ642245, MZ642259, , MN622787 and MZ642282 respectively.

Characterization of the bioactive components of the mushroom aqueous extracts showed