Effect of Neo Mineral Trioxide Aggregate and Hydroxyapatite Nanoparticulates on Odontogenic Differentiation and Proliferation of Human Dental Pulp Stem Cells

Abstract

Loss of pulp vitality results in a tooth more susceptible to structural, esthetic failure and secondary infection. Thus, the preservation of pulp vitality is considered an important challenge in order to maintain its function. The regenerative endodontic technology aims at preserving and regenerating the vitality and health of the dental pulp and restoring the physiologically functional dentition. The aim of the present study is to evaluate the effect of Neo MTA and hydroxyapatite nanoparticulates on odontogenic differentiation and proliferation of human dental pulp stem cells. The materials used in this study were Neo Mineral Trioxide Aggregate (NeoMTA 2) (Avalon Biomed, Texas, USA), prepared in nanoform at (Nanogate, Cairo, Egypt) and Nanohydroxyapatite (NHAP) (Nanogate, Cairo, Egypt). Human dental pulp tissues were obtained from three impacted third molars. Teeth were collected from three healthy patients aged from 18–20 years after written informed consent. Extraction of teeth was performed under sterile conditions in the department of Oral Surgery Department at Ain Shams University clinics under the approval of its ethics committee. MSCs were isolated from dental pulp tissue using enzyme digestion method by the following protocol: • The dental pulp tissue was minced into small pieces in Petri dish containing PBS (pH 7.4) and antibiotics.