Production and Evaluation of Local Radioimmunoassay System for Quantitative Estimation of Human Chorionic Gonadotropin in Human Serum

Abstract

This thesis aims to produce a valid technique with a low-cost radioimmunoassay system (RIA) to measure the glycoprotein so-called human chorionic gonadotropin (HCG) in human serum. Estimation of HCG in human serum is a very important tool in monitoring pregnancy and enables the early diagnosis, treatment and follow-up of gestational trophoblastic diseases.

Human chorionic gonadotropin is a glycoprotein hormone, that can be considered the wonder of today’s sciences, there are several variants of HCG each one play a critical function in pregnancy, among these variants regular HCG, has many functions; maintenance of corpus leutum that allows continuous production of progesterone during early pregnancy, blastocyte formation, hemochorial placentation, angiogenesis, and fetus protection against immunological attack from maternal blood. Hyperglycosylated HCG plays a critical role in implantation and invasion of the blastocyte into the uterus and has a major role in malignancy of most cancers, recently the new trend for the potential cure of cancer is the use of specific antibodies for hHCG.

The problem of nowadays market HCG immunometric assay kit is that almost all of them use monoclonal antibodies for HCG