PRODUCTION OF FIBRINOLYTIC ENZYME BY SOME ACTINOMYCETES

Abstract

In the present work, 7 actinomycete isolates from the Egyptian soil were obtained, identified and used in the screening programme planned for the detection of fibrinolytic enzymes to be used as a thrombolytic agent. Based on the preliminary results, further investigations were carried out on four actinomycetes namely, Streptomyces violatus 1, Streptomyces orientalis, Streptomyces violatus 2 and Streptosporangium albidum which showed relatively high fibrinolytic activity. Streptomyces orientalis proved to be the most potent of the investigated actinomycetes for the production of active extracellular fibrinolytic enzyme after about 4 days of incubation at 35°C using shaken cultures. For the optimisation of the enzyme productivity, a number of carbon sources were used as :glucose, fructose, galactose, maltose, sucrose and starch. When starch was substituted by galactose, 1.1-fold increase in activity and 2.18• fold in the protein content of the culture occurred. So, galactose was used as a carbon source ( 10 g/). The effect of nitrogen source on the biosynthesis of fibrinolytic enzyme was also studied. The utilisation of potassium nitrate, sodium nitrate, monosodium glutamate and ammonium sulphate showed a relatively low fibrinolytic activity ranging from 85% - 94% of the value obtained when casein was used as a nitrogen source. So, casein was used as a nitrogen source (5g/l).