Study of the Modulatory Effect of Amiloride on Doxorubicin Induced Neurotoxicity in Rats

Abstract

The aim of the current study was to test the hypothesis that the diuretic aniloride can protect against DOX-induced chemobrain in rats and to investigate the underlying mechanisms for the potential protection.

Experimental design: Rats were randomly divided into four groups (n=12): • The first group served as the control group and received the vehicles (saline and distilled water) • The second group (DOX) served as the disease group and received DOX dissolved in saline (2 mg/kg/week, i.p.) for 4 successive weeks. This dosing regimen was previously demonstrated to induce neurotoxicity in rats (Ali et al., 2020; El-Agamy et al., 2017; Kitamura et al., 2015). • The third group (DOX + AML) served as the treatment group and received AML dissolved in distilled water (10 mg/kg/day, i.p) concomitantly with DOX (2 mg/kg/week, i.p) for 4 successive weeks