CHEMICAL AND BIOLOGICAL EVALUATION OF SOME PALM OIL FRACTIONS

Abstract

This study aimed to investigate the effect of six different sources of oil and fat (100% corn oil, 100% butter, or100% margarine and three blends from margarine and butter at different percentages) on plasma lipid profile, lipid risk ratios and rats body weight when add in rat diet through a six weeks’ experimental period. Also the effect of them on internal organs (Liver, Kidney and Heart). Moreover, the free fatty acid (FFA) %, peroxide value (PV) and the fatty acid composition of the six types of oil and fat were determined. Thirty-six; white male Wistar albino rats, 50-70 gm were obtained from the animal house colony, National Research Centre, Giza, Egypt. All animals were housed in metal cages in a well-ventilated environment at 22 ± 3°C, 55 ± 5% humidity and 12h dark and light cycles. Animals received standard rat food pellets and water was provided ad-libitum throughout the experimental period. Rats were divided into six groups with 6 Rats in each group fed on variant types of oil and fat source as fat replacement within 6 weeks and classified as follow: Group 1 (G1): Rats were daily received (100% corn oil) as 10% of total diet orally and serve as (basal control diet). In other five groups the basal diet (100% corn oil) was replaced by other fat type and classified as follow: Group 2 (G2): Rats were daily received (100% butter) as 10% of total diet orally