MEMBRANE FATTYACIDS IN NORMAL AND EXPERIMENTALLY INDUCED DIABETES MELLITUS IN RATS

Abstract

Membrane fatty acids in normal and experimentally induced diabetes mellitus in rats were investigated. This study was carried out on 120, 12-16 weeks old, male rats and weighted 220-250gm. Rats were housed in a separate metal cages under the same constant environmental and nutritional condition through the period of investigation. The rats were divided into two main large equal groups, placed in individual cages and classified as follows: Group I: (Control group): Comprised 60 male rats, injected with citrate buffer only, served as control group. Group II: (Diabetic group): Included 60 male rats, injected with streptozotocin for diabetes induction, and served as experimental group. The experimental induction of diabetes in male rats was induced by a single intraperetinoel (i.p) injection of 50 mg / kg of streptozotocin freshly dissolved in citrate buffer, PH 4.5. Control rats received an equivalent amount of buffer alone. Random blood samples were collected by ocular vein puncture from all animal groups ( control and experimental group) five times at 2, 4, 6, 8 and l O weeks from the onset of diabetes induction. Blood samples were collected at the end of each experimental period and after overnight fasting in dry, clean, and screw capped tubes containing an anticoagulant solution, trisodium citrate 3.8 % with PH adjusted to 7.4 with citric acid (I vol. anticoagulant / 9 vol. blood) and plasma were separated by centrifugation at 2500 r.p.mfor 15 minutes. The clean, clear plasma was separated by Pasteur pipette and received in dry sterile sample tube, processed directly for glucose determination, then kept in a deep freeze at - 20°C until used for subsequent biochemical analysis. All plasma samples were analyzed for the following parameters: