SOME ANTIOXIDANTS LEVEL IN CHILDREN WITH CHRONIC LIVER DISEASES

Abstract

• Ten childern with chronic Wilson disease. • Fifteen apparently healthy children of matched age and sex severed as control group for this study. • Blood samples were obtained from subjected childern and collected in three tubes for each sample, one for LPO analysis, the second for vitamin A analysis and the third one for vitamin E analysis. • The blood smaples was collected in EDTA for vitamin E analysis. The all blood samples were transferred to the labe covered with aluminum foil were they centrifuged to obtain sera from the first and second tubes for LPO and vitamin A analysis and plasma was obtained from the third tube for vitamin E analysis. • Sera and plasma were kept at -20°C until analysis. • In addition to full history and clinical examination all of the studied individuals were subjected to the following investigations : complete blood count-total bilirubin- direct bilirubin- total protein- albumin-AST• ALT- ALP- prothrombin time hepatitis markers (HBsAg-HBcAb- HCV antibodies) and estimation of serum LPO, serum vitamin A and plasma vitamin E. • Abdominal ultrasonography. • Recto sigmoidoscopy and rectal snip was done for diagnosis of bilharzial liver diseases. • Liver biopsy in some cases when needed The results in our study revealed that : The mean values ± SD of LPO was (5.124± 0.231), (5.153± 0.371), (6.045 + 0,441), (5.167 ±0.407) (nmol/ml) in viral, bilharzial, GSD and Wilson groups. These values were significantly higher than (4.273+ 0.311) observed in the control ones.