Production of a genetically modified strain of bacteria Bacillus thuringiemisis

Abstract

This work has been carneri om u> order to ooii5intr a potent 6J suniri jciive j^jmsi The Egypitioin cottan Leaf "TJTJII £ iurvnUj. 7r>w,ird (his target, ihe 5-cndiMr>£ift crvsiaJ pitnejii genes rrWC (which encodes JTI imELUndal pruicm liijjdy specific to £ hrtoralu} jnd c>l ffAc *rl*c) were used HtntftU digested1 crvFC t^tf ^cied inffltfterf/rfflTIf S"c of UicshuCic v«(or pHT7593 dialed cry I Ag lig-ited info Snwl sue of pHT^* and inM 5iral ,inc of pHTNC3 The three pLisiiud con^cta were used to transform F- mit jtiaui JMK''J Colonies likely to contain recjmbmnnr pUsniidS were screened for the proJucLon afloxin proteins Positively idcnliUcd niiisfbrmasils produced the c\-pceled size protein detected br partidl purification of protein, and is tnjnaiicd upon Uypsin digestion. ProlCLiesirscisof clone J £i^lC-haitx)ring •was toxic to larvae ofS hnarniiir The pHT759j-beariji£ cy\C. pHT-bennngc^lAEaiidpHTOC3-harbonng-rrvlAg were transferred into Ihe Cry" Bid S; strain. PHT-beanng crylC was also transferred into ihe Ciyh AurjfaAj-HD^ (which contained only crylAc) native recipient using clcclroporalicn. The introduction of the c^lAg gcifr into Cry B14 resulted in the formation of bipyranudal crystals. The introduction of bolh rn> fenes \C. nnd 1 Agresulled in theinuJUpii£iiuon of bipyramidal cryslaisr Punhci, the introduction of ay\C into CTy' Jtw-HD73 shows muLhplicaUoninbipyraniidal cryTlal*