Generation of recombinant bioluminescent Escherichia coli for quantitative determination of bacterial adhesion
AlLuhaybi, Khalid Abdul Rahman; Alghaith, Ghadah Yazeed; Moneib, Nayera Ahmed; Yassien, Mahmoud;
Abstract
Bacterial adhesion to urinary catheter was evaluated by measuring the light emitted from a recombinant bioluminescent glycocalyx producer Escherichia coli strain. Generation of the bioluminescent strain was carried out by transforming the bacterial cells with pUCP18-GFP plasmid that contains a green fluorescence gene. Light emission measurement was closely correlated with the number of the adherent cells, giving a detectable signal from 1.2 X 10² cells. The efficiency of this assay was confirmed by testing the antiadherent effect of subinhibitory concentrations of ciprofloxacin with the aid of a model for in-vitro catheter colonization. There was no significant difference in the percentage reduction of adherent cells obtained by both light emission measurement and viable cell count techniques.
Other data
| Title | Generation of recombinant bioluminescent Escherichia coli for quantitative determination of bacterial adhesion | Authors | AlLuhaybi, Khalid Abdul Rahman; Alghaith, Ghadah Yazeed; Moneib, Nayera Ahmed; Yassien, Mahmoud | Keywords | Adherence Antiadherent effect;Catheter colonization;Recombinant bioluminescent E. coli;PUCP18-GFP plasmid | Issue Date | Jul-2015 | Journal | Pakistan Journal of Pharmaceutical Sciences | ISSN | 1011-601X | PubMed ID | 26142520 | Scopus ID | 2-s2.0-84937907568 |
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