Changes of cotton leaf worm haemocytes and esterases after exposure to compounds derived from urea and Rice Straw

Abstract

In the current study, the role of cotton leaf worm haemocytes and esterases in detoxification were recorded. The haemolymph was collected from the 6th instar larvae which treated with LC25, LC50, LC70 of three newly compounds extracted from wastes from natural origin, Cyano acetyl urea (CAU), Benzimidazolyl acetyl urea (BAU) from urea and Cyano acetyl urea (CAH) from rice straw as 4th instar larvae. The mean of total haemocytes counts in haemolymph of untreated 6th larval instar was 9430±35.78 cells/mm3. Data indicated an increase in the total haemocytes of treated ones with CAU, at LC25 and LC50, BAU at LC25 and CAH at LC25, LC50 of CAH the percentages of change were: 19.01, 14.31, 1.90, 21.00 and 14.55, respectively. In addition, the percentages of oenocytoid counts were increased in all concentrations for all compounds except for CAH (at LC70) as it did not change comparing control count. On contrast, data cleared reductions in granulocytes in all concentrations for all compounds except for BAU at LC50 as they increased slightly (15.12%). On the other hand, the results were showed great differences in number of zones of esterase activity and in substrate specificity between treated and untreated samples. For instance, in the case of α - naphthyl acetate, 36 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.28. While, in the case of b - naphthyl acetate, 39 esterase bands were detected in control and treated larval samples with Rf ranging between 0.01 to 0.92.