Efficacy of novel compounds derivative from urea and rice straw on some biological aspects, protein profiles and peroxidase of Spodoptera littoralis.

Abstract

A laboratory experiment was carried out to study some biological aspects (larval mortality, pupation, pupal weight, pupal duration, adult emergence and malformation pe rcentage) of 4th instar larvae after treated with three newly compounds extracted from wastes from natural origin, Cyano acetyl urea (CAU), Benzimidazolyl acetyl urea (BAU) from urea and Cyano acetyl hydrolyzate (CAH) from rice straw. In addition, the present study aimed to investigate the effect of the above mentioned compounds on protein profiles and peroxidase pattern. Data indicated that the response was positively-related to concentrations of the investigated extracts and larval mortality increased significantly with the increase in their concentrations. Pupation percentage and pupal weight declined dramatically through successive ascending concentrations. The pupal durations were increased significantly, while the percentage of the adult emergence was reduced significantly with ascending concentrations. The total inhibition was increased with increasing concentrations. On the other hand, some proteins in treated larvae were missed or expressed with less in density. Also, peroxidase pattern recorded in control and treated larval samples has 27 bands with Rf ranging between 0.04 and 0.84. The genetic distance between control and treated samples at LC25, LC50 and LC70 of (CAU), (BAU) and (CAH) were (0.71, 0.57, 0.71),(0.86, 0.69, 0.69) and (1.00,1.00,1.00), respectively.