Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndrome
Nermin M. Hamdy; Hala M. Ghanem; Mervat M. Mohamed; Fawzia A.A. Abd El-Rahman; Soliman, Ahmed F;
Abstract
In the pathogenesis of polycystic ovary syndrome (PCOS), despite the importance of autophagy and transforming growth
factor-β1 (TGF-β1), there is scarce information about their inter-relationship. Therefore, here we assessed the correlations
between beclin-1, a cornerstone in autophagy, and TGF-β1 in a letrozole-induced PCOS rat model. Accordingly, a total of
45 female adult albino Wistar rats were randomly assigned into control, vehicle (carboxymethyl cellulose), and PCO groups.
To establish the PCOS model, letrozole (1.0 mg/kg body wt., p.o.) was given once daily for three successive weeks.
Circulating levels of luteinizing hormone, follicle-stimulating hormone, testosterone, estradiol, and progesterone were
assayed along with ovarian total antioxidant capacity (TAC), protein carbonyl content (PCC), beclin-1 level, and TGF-β1
level. Ovarian morphology and ultrastructure were examined by hematoxylin and eosin staining and electron microscopy,
respectively. Compared to control groups, hormonal levels and ovarian morphology in the letrozole-exposed animals
indicated the successful construction of the PCOS model. Further, the PCO group exhibited an oxidative stress status
reflected by a significant decrease in ovarian TAC and a significant elevation in the PCC. Moreover, ovarian beclin-1 and
TGF-β1 levels were significantly increased with an enhancement of autophagy as revealed by electron microscopy. In
multiple linear regression models, only TGF-β1 was observed in the final model where it explained the 62.3% variability of
Beclin-1. In conclusion, the ovarian level of TGF-β1 might be a determinant factor of beclin-1 level in PCOS which may
provide new insight into the pathophysiology and therapy of the disease.
factor-β1 (TGF-β1), there is scarce information about their inter-relationship. Therefore, here we assessed the correlations
between beclin-1, a cornerstone in autophagy, and TGF-β1 in a letrozole-induced PCOS rat model. Accordingly, a total of
45 female adult albino Wistar rats were randomly assigned into control, vehicle (carboxymethyl cellulose), and PCO groups.
To establish the PCOS model, letrozole (1.0 mg/kg body wt., p.o.) was given once daily for three successive weeks.
Circulating levels of luteinizing hormone, follicle-stimulating hormone, testosterone, estradiol, and progesterone were
assayed along with ovarian total antioxidant capacity (TAC), protein carbonyl content (PCC), beclin-1 level, and TGF-β1
level. Ovarian morphology and ultrastructure were examined by hematoxylin and eosin staining and electron microscopy,
respectively. Compared to control groups, hormonal levels and ovarian morphology in the letrozole-exposed animals
indicated the successful construction of the PCOS model. Further, the PCO group exhibited an oxidative stress status
reflected by a significant decrease in ovarian TAC and a significant elevation in the PCC. Moreover, ovarian beclin-1 and
TGF-β1 levels were significantly increased with an enhancement of autophagy as revealed by electron microscopy. In
multiple linear regression models, only TGF-β1 was observed in the final model where it explained the 62.3% variability of
Beclin-1. In conclusion, the ovarian level of TGF-β1 might be a determinant factor of beclin-1 level in PCOS which may
provide new insight into the pathophysiology and therapy of the disease.
Other data
Title | Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndrome | Authors | Nermin M. Hamdy; Hala M. Ghanem; Mervat M. Mohamed; Fawzia A.A. Abd El-Rahman; Soliman, Ahmed F | Issue Date | May-2024 | Journal | Indian Journal of Experimental Biology | Volume | 62 | Issue | 5 | Start page | 332 | End page | 340 | ISSN | 00195189 09751009 |
DOI | 10.56042/ijeb.v62i05.1768 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.