Electrospun cellulose nanofibers immobilized with anthocyanin extract for colorimetric determination of bacteria
Hassan, Nada F; Khattab, Tawfik A; Fouda, Moustafa M G; Abu Zaid, Ahmed S; Aboshanab, Khaled;
Abstract
A novel smart biochromic textile sensor was developed by immobilizing anthocyanin extract into electrospun cellulose acetate nanofibers to detect bacteria for numerous potential uses, such as healthcare monitoring. Red-cabbage was employed to extract anthocyanin, which was then applied to cellulose acetate nanofibers treated with potassium aluminum sulfate as a mordant. Thus, nanoparticles (NPs) of mordant/anthocyanin (65-115 nm) were generated in situ on the surface of cellulose acetate nanofibrous film. The pH of a growing bacterial culture medium is known to change when bacteria multiply. The absorbance spectra revealed a bluish shift from 595 nm (purple) to 448 nm (green) during the growth of Gram-negative bacteria (E. coli) owing to the discharge of total volatile basic amines as secretion metabolites. On the other hand, the absorption spectra of a growing bacterial culture containing Gram-positive bacteria (L. acidophilus) showed a blue shift from 595 nm (purplish) to 478 nm (pink) as a result of releasing lactic acid as a secretion metabolite. Both absorbance spectra and CIE Lab parameters were used to determine the color shifts. Various analytical techniques were utilized to study the morphology of the anthocyanin-encapsulated electrospun cellulose nanofibers. The cytotoxic effects of the colored cellulose acetate nanofibers were tested.
Other data
Title | Electrospun cellulose nanofibers immobilized with anthocyanin extract for colorimetric determination of bacteria | Authors | Hassan, Nada F; Khattab, Tawfik A; Fouda, Moustafa M G; Abu Zaid, Ahmed S; Aboshanab, Khaled | Keywords | Anthocyanins bacterochromism;Bacteria sensor;Smart cellulose nanofibers | Issue Date | Feb-2024 | Journal | International Journal of Biological Macromolecules | Volume | 257 | Issue | 2 | ISSN | 01418130 | DOI | 10.1016/j.ijbiomac.2023.128817 | PubMed ID | 38103663 | Scopus ID | 2-s2.0-85180363542 |
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