ROLE OF BONE MARROW MESENCHAYMAL STEM CELLS (MSCs) ON RESTORATION OF FERTILITY IN MALE RATS AFTER EXPOSURE TO ENDOCRINE DISRUPTER

1Samiha M. Abd El Dayem, 2Mahmoud M. Arafa, 1Fatma M.A.Foda, 1Mona A. M. Helal, 3Fatma A. Abu Zahra, 1Nawal Z. Haggag ; Fouda, Fatma 


Abstract


This study aimed to examine the role of bone marrow mesenchymal stem cells (MSCs) in the restoration of fertility and reproductive functions in male rats after testicular failure induced butributyltin oxide (TBTO). Rats were divided into 5 groups: Group 1 (-ve control group) with no treatment; Group 2 (TBTO vehicle control) ; Group 3(TBTO group) rats were given orally TBTO 0.062 mg/kg b.wt in 1ml distilled H2O containing 50μ ethanol twice a week for 3 months ; Group 4 (TBTO and MSCs vehicles control) and Group 5(TBTO and MSCs treated group) rats were given (0.062) mg/kg b.wt TBTO twice a week for 3 months and then injected intravenously with a single dose of MSCs (3x106 cells in 0.5 ml phosphate buffer saline) per animal. After 5 months, male rats were allowed to mate with untreated females and then dissected. Serum samples were collected and testis sections were imune-histochemical stained for detection of CD44 positive and proliferating cell nuclear antigen (PCNA)-positive cells. Results showed that, TBTO caused significant reduction in the body weight, fertility rate and reproductive functions. The Seminiferous tubules appeared atrophied with obstructed lumen and oligospermia. However, the depletion of germ cells and decrease of PCNA-were noticed. Transplantation of MSCs markedly restored the reduction of body weights, fertility rate, serum testosterone, LH, FSH hormones, testicular enzymes, sperm counts and improved testicular DNA fragmentation. In the testicular tissue, CD44 positive cells were detected after 3months of MSCs transplantation with increase in the proliferative capacity (PCNA) stained cells. Even more, MSCs transplantation showed normal spermatogenesis process and complete recovery in germinal layers. In conclusion, MSCs transplantation can restore male fertility after disruption induced by tributyltin oxide in rats. If this protocol was proven to be functional in human, this would provide a new therapeutic concept for male infertility treatment.


Other data

Keywords Mesenchymal stem cells, Endocrine disrupters, Fertility index, Hormones, Testicular functions, Comet assay, Immunohistochemistry, Histology.
Issue Date 2015
Publisher International Journal of Pharmacy Review & Research
Journal International Journal of Pharmacy Review & Research Vol 5 Issue 3 158-169. 
URI http://research.asu.edu.eg/handle/123456789/2371


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