Synergistic renoprotective effect of a compiled branched-chain amino acids and Cymbopogon schoenanthus extract against experimentally induced oxido-nitrosative renal insult

Warda, Mohamad ; Ahmed-Farid, OmarAbdel-Hamed ; abd El-motelp, bosy ; Essa, EnaamAbdel-Mohsen 


Abstract


© 2018, Hainan Medical University. E-edition Published by Wolters Kluwer India Pvt. Ltd. All rights reserved. Objective: To better investigate the protective role of branched-chain amino acids (BCAAs) and Cymbopogon schoenanthus (CS) extract against the potassium dichromate (PDC)-induced oxido-nitrosative nephrotoxic insult in the experimental rat model. Methods: Thirty male rats were randomly divided into five equal groups: The 1st group served as control; the 2ndwas injected with a single dose of PDC (15 mg/kg b.w i.p.); the 3rd, 4th, and 5thgroups were respectively treated with BCAAs, CS, and their combination for 15 d prior to induction of renal insult via PDC single dose (15 mg/kg b.w s.c.). The experimental period was terminated in all groups 2 d after induction of renal insult. The harvested kdney samples were divided for biochemical assays and histological examination. Results: The PDC-induced nephrotoxic effect caused a depletion of renal oxidative scavengers glutathione, superoxide dismutase with consequent lipo-oxidative cellular membrane deterioration manifested by a rise in malonaldehyde, oxidized glutathione, myeloperoxidase and the concomitant increase in inflammatory response elements tumor necrosis factor μ, nitric oxide, and interleukin 1 β. Moreover, the comet assay and increased 8-hydroxy-2-deoxyguanosine proved an accelerated apoptotic DNA fragmentation. These local renal changes were met with global altered blood biochemistry. The BCAAs and CS or their compiled administration showed an ameliorative effect against PDC-induced nephrotoxic in a synergistic pattern. Conclusions: Both BCAAs and CS or their combined administration afford potential competitors against renal insult induced by polyvalent anion pollutants in experimentally studied animals model. As a route for novel drug discovery, further investigation should be attempted to optimize their augmenting reno-protecting potential.


Other data

Keywords Branched chain amino acids Cymbopogon schoenanthus Nephrotoxicity Comet assay Rats First author: Omar Abdel-Hamed Ahmed-Farid, Physiology Department, National Organization for Drug Control and Research, Giza 12553, Egypt. E-mail: ebntaimya@yahoo.com Corresponding author: Prof. Dr. Mohamad Warda, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt. Fax: 002-02 35725240 Tel: 002-02 35682195 Cell phone: (+20)1062368347 E-mail: maawarda@scu.eg; maawarda@hotmail.com 1. Introduction
Issue Date 30-May-2018
Publisher Asian Pacific Journal of Tropical Medicine
Journal Asian Pacific Journal of Tropical Medicine 
URI http://research.asu.edu.eg/handle/123456789/2414
DOI 5
https://api.elsevier.com/content/abstract/scopus_id/85047768451
342
11
10.4103/1995-7645.233182


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